4J0F
Crystal structure of 3-hydroxyacyl-CoA dehydrogenase from Caenorhadbitis elegans in P212121 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-06-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.557, 96.371, 114.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.660 - 2.200 |
| R-factor | 0.18968 |
| Rwork | 0.187 |
| R-free | 0.24574 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3had |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.894 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.660 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.091 | 0.366 |
| Number of reflections | 35658 | |
| <I/σ(I)> | 21 | 3.28 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.2 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 23% PEG 3350, 0.2M sodium chloride, 0.1M Bicine pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
