4IZ7

Structure of Non-Phosphorylated ERK2 bound to the PEA-15 Death Effector Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	93
Detector technology	CCD
Collection date	2011-12-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	P 21 21 2
Unit cell lengths	73.478, 205.334, 57.746
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.700 - 1.800
R-factor	0.18659
Rwork	0.185
R-free	0.22210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.362
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.6.0107)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.700
High resolution limit [Å]	1.800
Rmerge	0.080
Number of reflections	77047
<I/σ(I)>	24.8
Completeness [%]	99.1
Redundancy	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.2 M sodium malonate and 20% PEG 3350 , pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K