4IZ5
Structure of the complex between ERK2 phosphomimetic mutant and PEA-15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2011-06-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.801, 149.129, 98.869 |
| Unit cell angles | 90.00, 90.41, 90.00 |
Refinement procedure
| Resolution | 29.800 - 3.190 |
| R-factor | 0.24515 |
| Rwork | 0.243 |
| R-free | 0.29193 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0107) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.800 |
| High resolution limit [Å] | 3.190 |
| Rmerge | 0.079 |
| Number of reflections | 36572 |
| <I/σ(I)> | 21.4 |
| Completeness [%] | 98.6 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M Bis-Tris and 2.0 M ammonium sulfate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
