4IXV

Crystal structure of human Arginase-2 complexed with inhibitor 2d: {(5R)-5-amino-5-carboxy-5-[1-(4-chlorobenzyl)piperidin-4-yl]pentyl}(trihydroxy)borate(1-)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-06
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54178
Spacegroup name	P 42 21 2
Unit cell lengths	128.123, 128.123, 159.362
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.830 - 2.300
R-factor	0.1828
Rwork	0.180
R-free	0.23180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d3v
RMSD bond length	0.010
RMSD bond angle	1.430
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.830	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	54972
<I/σ(I)>	11.3	3.6
Completeness [%]	92.8	97.6
Redundancy	5.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	Crystals in condition H3 from Silver Bullet screen (Hampton Research) using Tacsimate in the reservoir, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K