4IXP
Crystal structure of Maternal Embryonic Leucine Zipper Kinase (MELK)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 135.449, 135.449, 153.652 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.847 - 2.749 |
R-factor | 0.2035 |
Rwork | 0.202 |
R-free | 0.23230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fe3 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.110 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.749 | 2.749 |
Rmerge | 0.059 | 0.473 |
Number of reflections | 22247 | |
<I/σ(I)> | 30 | 4.6 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.1 M Sodium Cacodylate, 0.7M Sodium Acetate, 2% PEG 400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |