4IXH
Crystal Structure of the Catalytic Domain of the Inosine Monophosphate Dehydrogenase from Cryptosporidium parvum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-14 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.185, 92.016, 92.139 |
| Unit cell angles | 90.00, 103.85, 90.00 |
Refinement procedure
| Resolution | 35.521 - 2.105 |
| R-factor | 0.1641 |
| Rwork | 0.162 |
| R-free | 0.21030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 3FFS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.104 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1161)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 83034 | |
| <I/σ(I)> | 8.2 | 2.1 |
| Completeness [%] | 99.2 | 96.5 |
| Redundancy | 4.2 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 289 | 0.1 M succinic acid pH 7.0, 15 %(w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
