4IUF

Crystal Structure of Human TDP-43 RRM1 Domain in Complex with a Single-stranded DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	110
Detector technology	CCD
Collection date	2012-04-03
Detector	RAYONIX MX300HE
Wavelength(s)	0.975
Spacegroup name	P 65 2 2
Unit cell lengths	71.110, 71.110, 101.663
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.336 - 2.752
R-factor	0.2116
Rwork	0.206
R-free	0.25860
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.751
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8_1059)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.750	2.750
Rmerge		0.554
Number of reflections	4328
<I/σ(I)>	43.5	8.6
Completeness [%]	10.0	100
Redundancy	17.8	18.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.12M CH3COONH4, 0.05M Bis-Tris, 16% PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K