4ISY
Crystal structure of IscS from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.814, 78.131, 92.774 |
| Unit cell angles | 94.54, 104.71, 98.57 |
Refinement procedure
| Resolution | 45.220 - 2.590 |
| R-factor | 0.18904 |
| Rwork | 0.186 |
| R-free | 0.24533 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lvm |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.833 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.590 | 2.590 |
| <I/σ(I)> | 11.15 | 3.61 |
| Completeness [%] | 94.3 | 90.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.2 ammonium sulfate, 0.1M bis-Tris pH 5.5, 5% glycerol and 10% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
