4IS9
Crystal Structure of the Escherichia coli LpxC/L-161,240 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | H 3 2 |
Unit cell lengths | 117.440, 117.440, 253.661 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.916 - 2.130 |
R-factor | 0.2173 |
Rwork | 0.216 |
R-free | 0.24730 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.705 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.170 |
High resolution limit [Å] | 2.130 | 5.780 | 2.130 |
Rmerge | 0.086 | 0.057 | 0.603 |
Number of reflections | 37998 | ||
<I/σ(I)> | 9.7 | ||
Completeness [%] | 100.0 | 99.5 | 100 |
Redundancy | 13.4 | 12.7 | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |