4IRZ
Crystal structure of A4b7 headpiece complexed with Fab Natalizumab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.610, 77.890, 217.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.619 - 2.840 |
R-factor | 0.2392 |
Rwork | 0.237 |
R-free | 0.28750 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.600 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 12.700 |
High resolution limit [Å] | 2.840 |
Number of reflections | 30297 |
Completeness [%] | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 6%PEG2000,0.1M Bicine pH9.0, 5%dioxane, 300mM NDSB-195, VAPOR DIFFUSION, HANGING DROP, temperature 277K |