4IRK
structure of Polymerase-DNA complex, dna
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.650, 57.080, 110.230 |
| Unit cell angles | 90.00, 94.92, 90.00 |
Refinement procedure
| Resolution | 35.180 - 2.320 |
| R-factor | 0.21137 |
| Rwork | 0.209 |
| R-free | 0.26236 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.781 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.450 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.102 | 0.530 |
| Number of reflections | 44132 | |
| <I/σ(I)> | 8.4 | 2.7 |
| Completeness [%] | 100.0 | 99.2 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 15% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 4.6 |
