4IRJ
Structure of the mouse CD1d-4ClPhC-alpha-GalCer-iNKT TCR complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-01 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9795706 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.283, 191.862, 151.598 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.350 - 3.000 |
| R-factor | 0.18808 |
| Rwork | 0.186 |
| R-free | 0.22844 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.189 |
| Refinement software | REFMAC (5.6.0104) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 21794 | |
| Completeness [%] | 98.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295.5 | 20% polyethylene glycol 4000, 0.2M sodium thiocyanate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K |
