4IRC
Polymerase-DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-26 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.140, 56.950, 110.810 |
Unit cell angles | 90.00, 93.53, 90.00 |
Refinement procedure
Resolution | 45.300 - 2.670 |
R-factor | 0.22696 |
Rwork | 0.225 |
R-free | 0.27504 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.431 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.810 |
High resolution limit [Å] | 2.670 | 2.670 |
Rmerge | 0.074 | 0.529 |
Number of reflections | 29306 | |
<I/σ(I)> | 10.7 | 2.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 2.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 15% MPD , pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |