4IRC
Polymerase-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.140, 56.950, 110.810 |
| Unit cell angles | 90.00, 93.53, 90.00 |
Refinement procedure
| Resolution | 45.300 - 2.670 |
| R-factor | 0.22696 |
| Rwork | 0.225 |
| R-free | 0.27504 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.431 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.810 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.074 | 0.529 |
| Number of reflections | 29306 | |
| <I/σ(I)> | 10.7 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 2.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 15% MPD , pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
