4IR9
Polymerase-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.760, 57.040, 110.700 |
| Unit cell angles | 90.00, 94.76, 90.00 |
Refinement procedure
| Resolution | 47.660 - 2.330 |
| R-factor | 0.21441 |
| Rwork | 0.211 |
| R-free | 0.27242 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.920 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.061 | 0.514 |
| Number of reflections | 44000 | |
| <I/σ(I)> | 10 | 2.2 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 20% MPD , pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
