4IP9
Structure of native human serum amyloid A1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-04 |
Detector | MAR CCD 165 mm |
Spacegroup name | H 3 2 |
Unit cell lengths | 93.850, 93.850, 130.520 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.460 - 2.500 |
R-factor | 0.25 |
Rwork | 0.249 |
R-free | 0.25840 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.860 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 7791 | |
<I/σ(I)> | 16.2 | 3 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 6.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 30% PEG2000 MME, 0.1M Tris (pH8.5) and 0.2 M Trimethylamine N-oxide, VAPOR DIFFUSION, HANGING DROP, temperature 295K |