4IP9
Structure of native human serum amyloid A1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-04 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 93.850, 93.850, 130.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.460 - 2.500 |
| R-factor | 0.25 |
| Rwork | 0.249 |
| R-free | 0.25840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.860 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 7791 | |
| <I/σ(I)> | 16.2 | 3 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 6.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 30% PEG2000 MME, 0.1M Tris (pH8.5) and 0.2 M Trimethylamine N-oxide, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
