4IP8

Structure of human serum amyloid A1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Detector	MAR CCD 165 mm
Spacegroup name	P 21 21 2
Unit cell lengths	68.591, 128.419, 50.199
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.754 - 2.193
R-factor	0.2065
Rwork	0.204
R-free	0.25610
Structure solution method	MAD
RMSD bond length	0.003
RMSD bond angle	0.665
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.190	2.190
Number of reflections	22939
<I/σ(I)>	24.4	2.8
Completeness [%]	98.3	92.6
Redundancy	9.9	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	2.0M (NH4)2SO4, 0.1M Hepes, 6% PEG400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K