4IP0
X-Ray Structure of the Complex Uridine Phosphorylase from Vibrio cholerae with Phosphate Ion at 1.29 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-13 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.81 |
Spacegroup name | P 1 |
Unit cell lengths | 63.671, 71.062, 87.908 |
Unit cell angles | 69.63, 72.56, 85.73 |
Refinement procedure
Resolution | 18.934 - 1.294 |
R-factor | 0.1731 |
Rwork | 0.172 |
R-free | 0.19970 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.216 |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 78.794 | 18.934 | 1.360 |
High resolution limit [Å] | 1.294 | 4.090 | 1.290 |
Rmerge | 0.028 | 0.266 | |
Total number of observations | 65746 | 127989 | |
Number of reflections | 327958 | ||
<I/σ(I)> | 14.2 | 23.2 | 2.3 |
Completeness [%] | 95.5 | 98.5 | 94.3 |
Redundancy | 3.8 | 6.1 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 291 | potassium phosphate, ammonium sulfate, sodium sulfate, pH 7.5, VAPOR DIFFUSION, temperature 291K |