4IOM
N10-formyltetrahydrofolate synthetase from Moorella thermoacetica with folate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 210r |
Spacegroup name | H 3 2 |
Unit cell lengths | 160.990, 160.990, 256.610 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 122.510 - 2.999 |
R-factor | 0.22073 |
Rwork | 0.213 |
R-free | 0.29094 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3QB6 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.498 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 122.510 | 3.110 |
High resolution limit [Å] | 2.999 | 2.999 |
Rmerge | 0.165 | |
Number of reflections | 25893 | |
<I/σ(I)> | 7.8 | |
Completeness [%] | 98.8 | 100 |
Redundancy | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 38 to 46% (w/v) saturated ammonium sulfate (AS), 1 mM DTT, and 1 to 3.5% (w/v) PEG 1000 or PEG 1450, in 50 - 75 mM KMB (pH 7.0 - 8.0), VAPOR DIFFUSION, HANGING DROP, temperature 298K |