4IMP
The missing linker: a dimerization motif located within polyketide synthase modules
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-25 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.815, 211.690, 101.742 |
| Unit cell angles | 90.00, 95.26, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.570 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mjs |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.149 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.570 | 2.600 |
| Rmerge | 0.056 | 0.555 |
| Number of reflections | 73458 | |
| <I/σ(I)> | 20.3 | 2.7 |
| Completeness [%] | 98.2 | 97.5 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 293 | 15 % (w/v) polyethylene glycol 3350, 0.2 M magnesium acetate, 30% glycerol (v/v), pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
