4IML
CrossFab binding to human Angiopoietin 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.755, 80.552, 158.266 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.133 - 2.931 |
| R-factor | 0.2387 |
| Rwork | 0.237 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.863 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.133 |
| High resolution limit [Å] | 2.795 |
| Number of reflections | 24225 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 17.5-20 %(w/v) PEG6000, 0.1 M citric acid, 0.2 M NDSB-221, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
