4IHO
Crystal structure of H-2Db Y159F in complex with chimeric gp100
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.93927 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 90.661, 146.011, 187.585 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.100 - 2.800 |
| R-factor | 0.25222 |
| Rwork | 0.249 |
| R-free | 0.30541 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ch1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.447 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.100 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 30269 | |
| Completeness [%] | 100.0 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.6-1.8M NH4SO4, 100mM Tris HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
