4IHL
Human 14-3-3 isoform zeta in complex with a diphoyphorylated C-RAF peptide and Cotylenin A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-10-06 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9778 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.280, 103.400, 112.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.400 - 2.200 |
R-factor | 0.2024 |
Rwork | 0.200 |
R-free | 0.24320 |
Structure solution method | MIR |
Starting model (for MR) | 4fj3 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.575 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.400 | 2.300 | |
High resolution limit [Å] | 2.200 | 12.000 | 2.200 |
Rmerge | 0.035 | 0.019 | 0.280 |
Number of reflections | 42870 | 318 | 5204 |
<I/σ(I)> | 21.65 | 53.62 | 4.66 |
Completeness [%] | 98.3 | 95.5 | 97.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1 M NA-ACETATE PH 7.0, 0.8 M NAH2PO4 AND 1.2 M K2HPO4, VAPOR DIFFUSION, SITTING DROP, temperature 277K |