4IHH
Chasing Acyl Carrier Protein Through a Catalytic Cycle of Lipid A Production
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 89.470, 93.225, 114.300 |
| Unit cell angles | 75.93, 73.81, 58.95 |
Refinement procedure
| Resolution | 35.886 - 2.132 |
| R-factor | 0.1787 |
| Rwork | 0.177 |
| R-free | 0.21640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.033 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.190 |
| High resolution limit [Å] | 2.132 | 5.830 | 2.150 |
| Rmerge | 0.088 | 0.036 | 0.640 |
| Number of reflections | 160164 | ||
| <I/σ(I)> | 8.7 | ||
| Completeness [%] | 96.1 | 99.1 | 85.6 |
| Redundancy | 5.4 | 5.8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 288 | 0.1 M MES pH 6.0, 0.2 M lithium sulfate, 20% PEG 4000, vapor diffusion, temperature 288K |
