4IHG
Chasing Acyl Carrier Protein Through a Catalytic Cycle of Lipid A Production
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 84.180, 89.310, 111.933 |
| Unit cell angles | 104.09, 92.58, 118.64 |
Refinement procedure
| Resolution | 28.433 - 2.890 |
| R-factor | 0.2059 |
| Rwork | 0.204 |
| R-free | 0.25290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.970 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1232) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.950 |
| High resolution limit [Å] | 2.890 | 7.860 | 2.900 |
| Rmerge | 0.087 | 0.031 | 0.190 |
| Number of reflections | 59734 | ||
| <I/σ(I)> | 6.2 | ||
| Completeness [%] | 98.3 | 97.9 | 91.1 |
| Redundancy | 3.9 | 4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 288 | 0.1 M MES pH 6.5, 0.2 M ammonium sulfate, 20% PEG 8000, vapor diffusion, temperature 288K |
