4IHG
Chasing Acyl Carrier Protein Through a Catalytic Cycle of Lipid A Production
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 84.180, 89.310, 111.933 |
Unit cell angles | 104.09, 92.58, 118.64 |
Refinement procedure
Resolution | 28.433 - 2.890 |
R-factor | 0.2059 |
Rwork | 0.204 |
R-free | 0.25290 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.970 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1232) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.950 |
High resolution limit [Å] | 2.890 | 7.860 | 2.900 |
Rmerge | 0.087 | 0.031 | 0.190 |
Number of reflections | 59734 | ||
<I/σ(I)> | 6.2 | ||
Completeness [%] | 98.3 | 97.9 | 91.1 |
Redundancy | 3.9 | 4 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 288 | 0.1 M MES pH 6.5, 0.2 M ammonium sulfate, 20% PEG 8000, vapor diffusion, temperature 288K |