4IGP
Histone H3 Lysine 4 Demethylating Rice JMJ703 apo enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 63 |
Unit cell lengths | 91.326, 91.326, 76.022 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.663 - 3.003 |
R-factor | 0.2301 |
Rwork | 0.227 |
R-free | 0.28660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oq6 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.507 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.000 |
Number of reflections | 6821 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | potassium phosphate monobasic, 25% (w/v) polyethylene glycol 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |