4IGO

Histone H3 Lysine 4 Demethylating rice Rice JMJ703 in complex with alpha-KG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 1W2B
Synchrotron site	BSRF
Beamline	1W2B
Detector technology	CCD
Collection date	2012-01-03
Detector	MAR CCD 165 mm
Wavelength(s)	1.0000
Spacegroup name	P 63
Unit cell lengths	90.518, 90.518, 76.048
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.892 - 2.400
R-factor	0.1952
Rwork	0.193
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oq6
RMSD bond length	0.009
RMSD bond angle	1.268
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	15149
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	potassium phosphate monobasic, 25% (w/v) polyethylene glycol 8000 , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	potassium phosphate monobasic, 25% (w/v) polyethylene glycol 8000 , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K