4IGG
Full-length human alpha-catenin crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 |
| Unit cell lengths | 145.629, 145.629, 139.081 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.210 - 3.660 |
| R-factor | 0.2187 |
| Rwork | 0.217 |
| R-free | 0.24130 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.990 |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | SHARP |
| Refinement software | TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 139.081 | 139.081 | 3.860 |
| High resolution limit [Å] | 3.658 | 11.570 | 3.660 |
| Rmerge | 0.029 | 0.491 | |
| Total number of observations | 6002 | 30931 | |
| Number of reflections | 36415 | ||
| <I/σ(I)> | 15.1 | 20.8 | 1.5 |
| Completeness [%] | 99.9 | 98.2 | 100 |
| Redundancy | 5.8 | 5.3 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.8 | 295 | 0.9 M Na/K Phosphate, 0.3 M Na Malonate, pH 6.8, VAPOR DIFFUSION, temperature 295K |
