4IGG

Full-length human alpha-catenin crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 32
Unit cell lengths	145.629, 145.629, 139.081
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.210 - 3.660
R-factor	0.2187
Rwork	0.217
R-free	0.24130
Structure solution method	SIRAS
RMSD bond length	0.008
RMSD bond angle	0.990
Data scaling software	SCALA (3.3.16)
Phasing software	SHARP
Refinement software	TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	139.081	139.081	3.860
High resolution limit [Å]	3.658	11.570	3.660
Rmerge		0.029	0.491
Total number of observations		6002	30931
Number of reflections	36415
<I/σ(I)>	15.1	20.8	1.5
Completeness [%]	99.9	98.2	100
Redundancy	5.8	5.3	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.8	295	0.9 M Na/K Phosphate, 0.3 M Na Malonate, pH 6.8, VAPOR DIFFUSION, temperature 295K