4IEN
Crystal Structure of Acyl-CoA Hydrolase from Neisseria meningitidis FAM18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 152.520, 152.520, 152.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.950 - 2.000 |
| R-factor | 0.16791 |
| Rwork | 0.167 |
| R-free | 0.19449 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vpm |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.590 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.950 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.229 | |
| Number of reflections | 79721 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290 | 100mM Tris pH8.5, 2M ammonium Phosphate, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
