4IEM
Human apurinic/apyrimidinic endonuclease (APE1) with product DNA and Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Collection date | 2001-07 |
| Wavelength(s) | 0.97956 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.639, 74.090, 112.138 |
| Unit cell angles | 90.00, 111.98, 90.00 |
Refinement procedure
| Resolution | 29.643 - 2.394 |
| R-factor | 0.2099 |
| Rwork | 0.208 |
| R-free | 0.24620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.918 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.393 |
| Number of reflections | 56727 |
| Completeness [%] | 90.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 50 mM MES pH 6.0, 200 mM LiSO4, and 25% mPEG 2K, vapor diffusion, hanging drop, temperature 298K |
