4I8C
X-ray structure of NikA in complex with Ni-(L-His)2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 62 |
| Unit cell lengths | 158.630, 158.630, 135.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.500 - 2.503 |
| R-factor | 0.1907 |
| Rwork | 0.189 |
| R-free | 0.23390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dp8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.091 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.500 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 66527 | |
| <I/σ(I)> | 2.34 | |
| Completeness [%] | 98.0 | 96.4 |
| Redundancy | 2.58 | 2.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | 1.5M Ammonium sulfate, 0.1M sodium acetate, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
