4I7B
Siah1 bound to synthetic peptide (ACE)KLRPV(ABA)MVRPTVR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-28 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.745, 87.913, 59.884 |
Unit cell angles | 90.00, 100.59, 90.00 |
Refinement procedure
Resolution | 35.700 - 3.000 |
R-factor | 0.18951 |
Rwork | 0.186 |
R-free | 0.26051 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2A25 CHAIN A |
RMSD bond length | 0.005 |
RMSD bond angle | 0.907 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.077 | 0.600 |
Number of reflections | 8152 | |
<I/σ(I)> | 25.1 | 2.5 |
Completeness [%] | 99.5 | 97.7 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 10% PEG6000, 5% MPD, 100 mM HEPES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |