4I6Y
3-hydroxy-3-methyl (HMG) Coenzyme A Reductase bound to R-Mevalonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 225.806, 225.806, 225.806 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.790 - 1.450 |
| R-factor | 0.17472 |
| Rwork | 0.174 |
| R-free | 0.19431 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.502 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 160384 | |
| Completeness [%] | 97.9 | 92.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.2M ammonium sulfate, 75mM sodium citrate, 100 mM sodium ADA, with microseeding, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
