4I60
Crystal structure of avidin - biotinylruthenocene complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-10 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.8015 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 60.220, 60.220, 62.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.300 - 2.500 |
R-factor | 0.16711 |
Rwork | 0.165 |
R-free | 0.21103 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vyo |
RMSD bond length | 0.018 |
RMSD bond angle | 2.264 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.360 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.132 | 0.505 |
Number of reflections | 4301 | |
<I/σ(I)> | 19.49 | 4.75 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 9.07 | 8.59 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 35% pentaerythritol propoxylate, 0.05M HEPES, 0.2M KCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |