4I27
Ternary complex of mouse TdT with ssDNA and incoming nucleotide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.8726 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.940, 95.900, 47.230 |
Unit cell angles | 90.00, 107.75, 90.00 |
Refinement procedure
Resolution | 47.950 - 2.600 |
R-factor | 0.1741 |
Rwork | 0.171 |
R-free | 0.23300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jms |
RMSD bond length | 0.010 |
RMSD bond angle | 1.090 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.950 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.137 | 0.461 |
Number of reflections | 12246 | |
<I/σ(I)> | 2.5 | |
Completeness [%] | 99.5 | 99.9 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG4000, 20% isopropanol, 100 mM sodium citrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |