4I1L
Structural and Biological Features of FOXP3 Dimerization Relevant to Regulatory T Cell Function
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 37.130, 37.130, 135.672 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.810 - 2.100 |
| R-factor | 0.238 |
| Rwork | 0.236 |
| R-free | 0.28900 |
| Structure solution method | MAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.889 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.064 | 0.511 |
| Number of reflections | 6111 | |
| <I/σ(I)> | 30.86 | 3.75 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 5.2 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 291 | 20MM HEPES, PH 7.3, 1MM TCEP, 0.5% CHAPS, 100MM NACL, 100MM NAAC PH 4.5, ~1.8M MGSO4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
