4I1H
Structure of Parkin E3 ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-02 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 87.110, 133.930, 66.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.971 - 2.000 |
| R-factor | 0.18239 |
| Rwork | 0.181 |
| R-free | 0.21537 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4i1f |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.842 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.971 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.398 | |
| Number of reflections | 21783 | |
| <I/σ(I)> | 12.1 | 3.6 |
| Completeness [%] | 82.0 | 36.3 |
| Redundancy | 6.1 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 283 | 0.1 M Tris, pH 6.5, 0.2 M sodium chloride, 25% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 383K |
