4I1F
Structure of Parkin-S223P E3 ligase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.2831, 1.2699 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 86.955, 133.155, 65.385 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.810 - 1.580 |
| R-factor | 0.20706 |
| Rwork | 0.205 |
| R-free | 0.24528 |
| Structure solution method | MAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.260 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.000 | 1.670 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.990 | |
| Number of reflections | 52033 | |
| <I/σ(I)> | 11 | 1.6 |
| Completeness [%] | 98.5 | 99.8 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 283 | 0.1 M HEPES, pH 7.5, 20% PEG4000, 10% isopropanol, 10 mM barium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
