4I13

Nanobody ca1697 binding to the DHFR.folate binary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-03
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.87260
Spacegroup name	P 21 21 21
Unit cell lengths	49.760, 58.050, 114.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.611 - 1.600
R-factor	0.1554
Rwork	0.154
R-free	0.18030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.051
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.690
High resolution limit [Å]	1.600	1.600
Number of reflections	44517
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	17% w/v PEG 1500, 10% v/v MPD, 50mM PIPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K