4I0U
Improved structure of Thermotoga maritima CorA at 2.7 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 116.250, 151.500, 143.360 |
| Unit cell angles | 90.00, 98.88, 90.00 |
Refinement procedure
| Resolution | 38.305 - 2.700 |
| R-factor | 0.2312 |
| Rwork | 0.228 |
| R-free | 0.28910 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.618 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.500 | 2.731 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 121600 | |
| Completeness [%] | 90.5 | 77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 100mM MGCL2, 10% PEG 1500, 1% DDM, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
