4HZU
Structure of a bacterial energy-coupling factor transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97947 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.971, 148.690, 154.574 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.685 - 3.530 |
| R-factor | 0.2432 |
| Rwork | 0.241 |
| R-free | 0.29170 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.823 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.685 | 3.660 |
| High resolution limit [Å] | 3.530 | 3.530 |
| Rmerge | 0.096 | 0.888 |
| Number of reflections | 22601 | |
| <I/σ(I)> | 13.9 | 1.4 |
| Completeness [%] | 96.9 | 97.9 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 291 | 17% (w/v) polyethylene glycol 2000, 16% (v/v) glycerol, 100 mM Tris buffer (pH 8.2), and 100 mM magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
