4HYO
Crystal Structure of MthK Pore
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2011-12-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 |
Unit cell lengths | 44.035, 63.452, 63.477 |
Unit cell angles | 90.03, 89.99, 89.99 |
Refinement procedure
Resolution | 44.864 - 1.650 |
R-factor | 0.1646 |
Rwork | 0.164 |
R-free | 0.18090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ldc |
RMSD bond length | 0.010 |
RMSD bond angle | 1.173 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.710 |
High resolution limit [Å] | 1.650 | 3.550 | 1.650 |
Rmerge | 0.042 | 0.027 | 0.397 |
Number of reflections | 79424 | ||
<I/σ(I)> | 14.5 | ||
Completeness [%] | 96.3 | 99.1 | 93.8 |
Redundancy | 3.2 | 3.3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 3.5-4.0M 1,6-hexanediol, 100mM MES , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |