4HYJ
Crystal structure of Exiguobacterium sibiricum rhodopsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 96.099, 96.099, 124.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 69.169 - 2.300 |
| R-factor | 0.1775 |
| Rwork | 0.175 |
| R-free | 0.21490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Homology model based on PDB ENTRY 3DDL |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.401 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.170 | 2.420 |
| High resolution limit [Å] | 2.150 | 2.300 |
| Rmerge | 0.054 | 0.334 |
| Number of reflections | 35982 | |
| <I/σ(I)> | 12 | 3.7 |
| Completeness [%] | 98.0 | 96.7 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Cubic phase crystallization | 295 | Cubic phase crystallization, temperature 295K |
