4HX9
Designed Phosphodeoxyribosyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-03-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 218.454, 218.454, 218.454 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.620 - 2.680 |
| R-factor | 0.19801 |
| Rwork | 0.195 |
| R-free | 0.24953 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f8y |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.900 | 2.820 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.509 | |
| Number of reflections | 47987 | |
| <I/σ(I)> | 2.8 | |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 293 | NH4SO4 1.2 M, 0.1 M HEPES at pH 7.7, PEG400 2%, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
