4HW7
Crystal structure of FMS kinase domain with a small molecular inhibitor, PLX647-OME
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1159 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 62.964, 62.964, 182.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.900 - 2.900 |
R-factor | 0.222 |
Rwork | 0.220 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.891 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.500 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 8735 | |
Completeness [%] | 99.6 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 20% PEG8K, 0.2M MgCl and 0.1M Tris, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |