4HV8
Crystal Structure of H2Db-H155A-NPM6I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.956 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 83.920, 72.930, 86.380 |
| Unit cell angles | 90.00, 103.11, 90.00 |
Refinement procedure
| Resolution | 72.930 - 2.000 |
| R-factor | 0.1835 |
| Rwork | 0.181 |
| R-free | 0.23580 |
| Starting model (for MR) | 3cpl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.100 | |
| High resolution limit [Å] | 2.000 | 10.000 | 2.000 |
| Rmerge | 0.067 | 0.019 | 0.483 |
| Number of reflections | 68339 | ||
| <I/σ(I)> | 13.5 | 37.51 | 2.84 |
| Completeness [%] | 99.3 | 95.4 | 99.9 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1M Tris HCl, 0.2M LiSO4, 25-30% PEG 8000, pH 8.5, vapor diffusion, hanging drop, temperature 298K |
