4HTJ

Crystallographic structure of the membrane-proximal ectodomain of the human receptor-type protein-tyrosine phosphatase phogrin at pH 4.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-16
Detector	ADSC QUANTUM 270
Wavelength(s)	0.91840
Spacegroup name	P 61 2 2
Unit cell lengths	54.742, 54.742, 149.147
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.400 - 2.011
R-factor	0.204
Rwork	0.203
R-free	0.23770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qt7
RMSD bond length	0.019
RMSD bond angle	2.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.030
High resolution limit [Å]	2.000	5.390	2.000
Rmerge	0.063	0.042	0.791
Number of reflections	9208
<I/σ(I)>	13.7
Completeness [%]	95.4	81.9	61.9
Redundancy	12.5	11.4	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.2 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6, 25 % w/v Polyethylene glycol 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K