4HRE

Crystal Structure of p11/Annexin A2 Heterotetramer in Complex with SMARCA3 Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Wavelength(s)	1.07500
Spacegroup name	P 1
Unit cell lengths	50.791, 74.346, 154.466
Unit cell angles	90.14, 96.56, 108.37

Refinement procedure

Resolution	47.335 - 2.785
R-factor	0.2567
Rwork	0.249
R-free	0.29600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1W7B and 4HRH
RMSD bond length	0.008
RMSD bond angle	1.241
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.335
High resolution limit [Å]	2.785
Number of reflections	53292
Completeness [%]	95.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.7	293	0.1 M citric acid, 25% PEG 3350, pH 3.7, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K