4HQU
Crystal structure of human PDGF-BB in complex with a modified nucleotide aptamer (SOMAmer SL5)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979176 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.710, 59.710, 167.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.270 - 2.200 |
| R-factor | 0.227 |
| Rwork | 0.225 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CHAIN A of 3MJG |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.882 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.270 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.040 | 0.578 |
| Number of reflections | 15629 | |
| <I/σ(I)> | 25.33 | 3.4 |
| Completeness [%] | 96.8 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | PROPLEX D8: 0.1 M MAGNESIUM ACETATE, 0.1 M SODIUM CACODYLATE PH 6.5, 15% PEG 6000, VAPOR DIFFUSION, TEMPERATURE 289K, VAPOR DIFFUSION, SITTING DROP |
