4HPO
Crystal structure of RV144-elicited antibody CH58 in complex with V2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2012-03-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.594, 75.804, 54.688 |
| Unit cell angles | 90.00, 111.99, 90.00 |
Refinement procedure
| Resolution | 24.806 - 1.694 |
| R-factor | 0.1866 |
| Rwork | 0.185 |
| R-free | 0.21080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3H0T 3ujj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.105 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.694 | 1.694 |
| Rmerge | 0.387 | |
| Number of reflections | 53546 | |
| <I/σ(I)> | 1.88 | |
| Completeness [%] | 90.7 | 56.1 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 14% (w/v) PEG 8000, 2% (v/v) MPD, 0.1 M imidazole pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
