4HPG
Crystal structure of a glycosylated beta-1,3-glucanase (HEV B 2), an allergen from Hevea brasiliensis
Replaces: 3EM5Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-15 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.176, 89.778, 101.542 |
Unit cell angles | 90.00, 113.59, 90.00 |
Refinement procedure
Resolution | 25.208 - 2.536 |
R-factor | 0.1948 |
Rwork | 0.193 |
R-free | 0.24050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cyg |
RMSD bond length | 0.011 |
RMSD bond angle | 1.485 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.500 | 25.210 | 2.640 |
High resolution limit [Å] | 2.500 | 7.910 | 2.500 |
Rmerge | 0.073 | 0.103 | 0.134 |
Number of reflections | 48122 | ||
<I/σ(I)> | 5.1 | 4.2 | 5.1 |
Completeness [%] | 96.6 | 96.9 | 80.7 |
Redundancy | 4 | 4 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 0.2M ammonium acetate, 0.1M trisodium citrate dihydrate, 30%(W/V) polyethylene glycol 4000., pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |